Workshop: Workshop on Fundamentals of Mass Spectrometry-based Proteomics for Beginners        

Date: August 23-25, 2018

Timings: 9:00 AM-6:30 PM

Venue: Center for Systems Biology and Molecular Medicine, Yenepoya Research Centre, Yenepoya (Deemed to be University)


Schedule


Workshop Program

Day 1:

Tutorial Lectures

  • Introduction to proteomics
  • Fractionation strategies
  • Sample preparation strategies
  • Day 2:

    Tutorial Lectures

  • Basics of LC-MS/MS analysis
  • LC-MS/MS operation
  • Key concepts of Mass spectrometry data analysis
  • Day 3:

    Tutorial Lectures

  • Quantitative proteomics
  • Validation of proteomics data
  • Biological interpretation of proteomics data
  • TMT labeling
  • Hands on training

  • Sample preparation I (In-gel and in-solution digestion)
  • Hands on training

  • Sample preparation II (peptide extraction, C18 clean-up)
  • Data analysis for qualitative proteomics
  • Hands on training

  • Data analysis for quantitative proteomics